114 BACTERIAL POISONS. 



bacilli. Among the latter was one which was somewhat 

 longer and slightly thicker than the bacillus of mouse- 

 septicaemia. In the subcutaneous cellular tissue he found 

 this bacillus alone, but could not detect it in the blood, 

 muscles, or nerves. Heating the soil for an hour rendered 

 the inoculations with it harmless. In cultures, Nicolaier 

 was unable to separate this bacillus from other germs, but 

 inoculations with mixed cultures produced tetanus. In the 

 same year. Carle and Ratoxe induced tetanus in lower 

 animals by inoculations with matter taken from a pustule 

 on a man just dead from tetanus. In 1886, Rosenbach 

 made successful inoculations on animals with matter taken 

 from a man who had died from tetanus consequent upon 

 gangrene from frozen feet. With bits of skin taken from 

 near the line of demarcation, he inoculated two guinea-pigs 

 on the thigh ; tetanic symptoms set in within twelve hours, 

 and one animal died within eighteen, and the other within 

 twenty-four hours. The symptoms corresponded exactly 

 with those observed in the " earth tetanus " of Nicolaier, 

 and the same bacillus was found. With mixed cultures of 

 this, RosENBACH was also able to cause death by tetanus 

 in animals. Bedmer had under observation a man who 

 died from lockjaw following the sticking of a splinter of 

 wood under his finger-nail. Inoculations of mice and 

 rabbits with some of the dirt found on the wood led to 

 tetanus. The same observer saw a boy die from this dis- 

 ease following an injury to the foot from a sharp piece of 

 stone. White mice inoculated with matter from the wound, 

 and those inoculated with dirt taken from the boy's play- 

 groimd, died of tetanus. The bacillus of Nicolaier v:as 

 again detected. Giordano reports the case of a man who 

 fell and sustained a complicated fracture of the arm. He 

 remained on the ground for some hours, and when assist- 

 ance came the muscles and skin were found torn and the 

 wounds filled with dirt. On the fifth day he showed symp- 

 toms of tetanus, from which he died on the eighth day. 

 Inoculations and examinations for the bacillus were again 

 successful. Ferrari also made successful inoculat-ons 

 with the blood taken during life from a woman with 



