TUBERCULOSIS. 123 



and there iu the lungs and liver, clinging to the vessel walls 

 for many days without inducing any marked changes in the 

 latter. After a time, however — earliest in the lung, later, 

 as a rule, in the liver — a cell proliferation occurs in the 

 vicinity of these dead germs, which leads to the formation 

 of new multiple nodular structures bearing a striking mor- 

 phological resemblance to miliary tubercles. There is iu 

 them, however, no tendency to cheesy degeneration and no 

 evidence of proliferation of the bacilli, but rather a steady 

 diminution in their number. It seems to us that the new 

 structures originate in a proliferation of the vascular endo- 

 thelium under the stimulus of the dead and disintegrating 

 germs." 



Maffucci finds that cultures of the tubercle bacillus 

 (from a mammal), when grown from one to six months on 

 glycerin, blood-serum, or liquid blood-serum, and then 

 sterilized by being repeatedly heated to from 65° to 70°, 

 produces in guinea-pigs, when employed subcutaneously, a 

 progressive marasmus, which terminates fatally within from 

 fourteen days to five or six months. He also finds that 

 eggs inoculated with sterilized cultures of the chicken tuber- 

 culosis bacillus produce chickens which are feeble and soon 

 die of emaciation. In neither the guinea-pigs nor chickens 

 could he find any tubercles. This author, unfortunately, 

 does not state positively whether the bacilli employed in his 

 experiments on guinea-pigs were obtained from man or some 

 other mammal. 



Crook SHANK and Heeroun report, the isolation of a 

 ptomaine and an albumose not only from artificial cultures 

 of the bacillus, but also from bovine tuberculous tissue. 

 The ptomaine is reported as causing an elevation of tem- 

 perature in tuberculous, and a depression in healthy, ani- 

 mals. " The albumose, whether obtained from pure culti- 

 vations of the bacillus, or from tuberculous tissue, produced 

 a marked rise of temperature in tuberculous guinea-pigs. 

 On the other hand, in an experiment tried on a healthy 

 guinea-pig, there was an equally well-marked fall of tem- 

 perature," 



