SUPPUKATION. 131 



backs of rabbits with, antis'jptic precautious, aud tiio ends 

 of the tubes broken off subcutaneously. 



After Irom two to three days the tubes were removed 

 and ibund to contain, in addition to some of the proteid, 

 several millimetres of fibrinous pus, which was examined 

 microscopically and by the preparations of cultures, which 

 invariably remained sterile. Tlie proteid of the Ebeeth 

 bacillus . was found to have specially marked pyogenetic 

 properties. 



Similar experiments were made with the following crys- 

 talline subsfamces : the butyrate and valerianate of ammo- 

 nia (each 1 per cent, solution), trimethylamin (2 per cent.), 

 ammonia (2 per cent.), leucin, tyrosin and glycocol (1 per 

 cent.), urea (6 per cent.), and urate of ammonia and skatol 

 (1 per cent.). Glycocol and Icuchi only were found to have 

 tiie chemotiictic action, and with these this action was l)ut 

 slight compared with that of the bacterial proteids. 



riie next experiments were made witn the object of 

 ascertaining whether or not proteids similar to those derived 

 from the bacteria would cause a like effect. The bacterial 

 cellular proteids resemble very closely vegetable casein 

 some of which was prepared from wheat gluten and tested 

 as above. This proteid was found to be possessed of 

 marked chemotactic properties. The subcutaneous injec- 

 tion of sterilized preparations of wheat-flour and ground 

 peas were also found to cause suppuration. JSegative 

 results were obtained with starch and solutions of disodium 

 hydrie phosphate. From this it is concluded that the active 

 agent in tlie flour is its casein. 



Peptone was employed without effect, while gelatin was 

 found to act energetically. Alkaline albuminates were 

 prepared from muscle, liver, lungs, and kidney by treating 

 hnely divided portions of these organs with potash and pro- 

 ceeding as in the preparation of the bacterial proteids. All 

 of these caused the formation of pus, and the preparations 

 from the liver were found to be specially potent. 



Similar preparations from blood and egg-yolk were 

 active, while those from fibrin and the white of egg had no 

 effect. Hemi-albumose was also found to be active, and 



