GERMICIDAL PKOTEIDS OF THE BLOOD. 155 



activity, while dialysis against 0.75 per ceut. salt solution 

 does not. In the diffusate there is no germicidal substance. 

 The loss by dialysis with water must be due to the with- 

 drawal of the inorgauic salts of the serum. 



(5) The same is shown to be the case when the serum is 

 diluted with water and when it is diluted with the salt 

 solution. In the former the germicidal action is destroyed, 

 while in the latter it is not. 



(6) The inorganic salts have in and of themselves no 

 germicidal action. They are active only in so far as they 

 affect the normal properties of the albuminates of the serum. 

 The germicidal properties of the serum reside in its proteid 

 constituents. 



(7) The difference in the effects of the active serum and 

 that which has been heated to 55° is due to the altered 

 condition of the albuminates. This difference may possibly 

 be a chemical one (due to changes within the molecule), or it 

 may be due to alterations in mycelial construction. The 

 albuminates act on the bacteria only when the former are 

 in an "active state." 



Halliburton has prepared from the lymphatic glands 

 a globulin which he designates as cell-globulin /5, and which 

 agrees with fibrin ferment in inducing coagulation in plasma. 

 Hankin has tested the germicidal properties of this cell- 

 globulin. His experiments have been conducted in the 

 following manner : The lymphatic glands (in later experi- 

 ments the spleen, also) of a dog or cat are freed as much 

 as possible from fat and connective tissue, then finely 

 divided, and extracted with a dilute solution of sodium 

 sulphate (one part of a saturated sodium sulphate solution 

 + nine parts of water). The cell-globulin passes into 

 solution, while the other proteids are but sparingly soluble. 

 After twenty-four hours the fluid is filtered and mixed 

 with an excess of alcohol. The voluminous precipitate 

 containing the cell-globulin is collected on a filter and 

 washed with absolute alcohol. For use, a part is dissolved 

 in water and a small quantity of a bouillon culture of the 

 anthrax bacillus added. Plate cultures are made along 

 with control plates from time to time, and in this way the 



