CHAPTER XIV 



THE CAPSULATED GROUP 

 Members — 



Varieties of Bacillus mucosus capsulatus. 



B. lactis aero genes. 

 Reference — 



Perkins, Journal oj Infectious Diseases^ 1904, No. n. 



Inoculate agar-slants from stock-cultures of B. mucosus 

 capsulatus (Friedlander's pneumo-bacillus) and B. lactis 

 aero genes. ^ 



1. Routine study. — Observe particularly the viscous 

 condition of cultures on solid media, the consistency of 

 liquid media, and the gas-formation on potato. 



2. Special study A. — Staining of capsules from 24- 

 hour-old milk-cultures (see p. 77). 



3. Special study B. — Intraperitoneal inoculation of 

 rabbit with B. mucosus capsulatus. 



Method oj intraperitoneal inoculation. — The rabbit is 

 held in the same manner as described in Chap. XII, 

 The hair is clipped close over the left lower abdominal 

 quadrant. Then (after washing with mercuric chlorid 

 1 : 1000 and alcohol) pass the needle at first beneath the 

 skin, then, holding it at about a right angle to the abdomi- 

 nal surface, carefully press it through the abdominal wall, 

 which is usually made tense by the resistance of the 

 animal. Successful passage of the abdominal wall can 

 be felt by the sudden loss of resistance to the needle's 

 pressure. Then make the injection, and rapidly with- 



' B. lactis aerogenes, if studied in the colon group, need not 

 be studied again - 



92 



