CHAPTER XXI 

 THE ACTINOMYCES GROUP 

 Inoculate agar-slants from Actinomyces bovis (homi- 

 nis) and Actin. asteroides. 



References — 



Stokes, American Journal of the Medical Sciences, Novem- 

 ber, 1904. 

 Wright, Jour, of Med. Research, May, 1905. 



1. Special study A. — Transfer from agar-slants to 

 broth and potato only, and make descriptions and 

 stained preparations as usual. 



2. Special study B. — Suspend a small amount of the 

 potato-culture in a physiological salt solution, and 

 examine under the low power. 



3. Special study C. — 



a) Examine a sample of actinomycotic tissue (bovine) 

 in the fresh state, for so-called "sulphur granules." 

 Crush some in salt solution under a cover-slip and search 

 for "clubs," using the low and high power dry lenses. 



h) Crush others, dry, fix, and stain by Gram, counter- 

 stain with eosin or Bismarck brown. 



c) Write a full description of the biology of the organ- 

 ism, illustrated by drawings. 



d) AVhat other actinomycetes have been described 

 as pathogenic? Give their names and the diseases 

 which they produce. 



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