ANAEROBIC GROUP OF BACILLI 



109 



d) Open the faucet (b) of the gas-generator (c), con- 

 taining zinc and hydrochloric acid. The hydrogen gas 

 generated passes through two jars, one of which con- 

 tains concentrated sulphuric acid (d), the other a 10 per 

 cent, solution of sodium hydrate (e). Gradually the 

 Novy jar is filled with hydrogen gas, which can be tested 

 by holding a culture-tube over the opening (/), and then 

 over a burning match or gas flame. As long as any 

 detonation takes place the hydrogen is still mixed with 



— a, 



Fig. 34 

 Anaerobic Cultivation in Hydrogen Gas 

 a. Novy jar e. Sodium hydrate solution 



A. Glass cock /. Opening of Novy jar 



c. Gas generator g. Stopper 



d. Sulphuric acid 



atmospheric oxygen. When finally the hydrogen in the 

 Novy jar is pure, close it off by turning the stoppers (g) 

 and (b), and place it in the incubator. The whole pro- 

 cess occupies about 10 or 15 minutes. 



5. Special study C. — Inoculation of a rabbit with 

 B. aerogenes capsulatus. 



a) Shave the ear of the rabbit. 



