APPENDIX I 

 SPECIAL MEDIA 



STANDARD METHOD OF PREPARING BROTH, NUTRIENT 



GELATIN AND NUTRIENT AGAR 

 {Report of American Public Health Assocciation,\o\. XXX, 1905.) 



9. Heat over boiling water (or steam) for 30 minutes. 



10. Restore loss by evaporation. 



1 1 . Titrate after boiling one minute to expel carbonic acid. 



12. Adjust reaction to -f i.o % by adding normal hydrochloric acid or sodium 



hydrate d>s required. 



125 



