SPFXIAL MEDIA 129 



Mac Conkey's bile-salt broth. — 



Sodium taurocholate 0.5^ 



Peptone 2.0^ 



Glucose 0.5^ 



are dissolved in beef-broth by boiling; then filter and add 

 litmus-solution. 



Parietti's solution— 



Carbolic acid S cc. 



Hydrochloric acid 4 c.c. 



Water 100 c.c. 



Eisner's medium. — i pound of sliced potatoes is grated 

 with a liter of water, and the juice expressed. After filtering 

 for 24 hours, add i per cent, peptone, i per cent, potassium 

 iodid, and 10 per cent, gelatin. 



Glycerinated potato. — Prepare potatoes in the ordinary 

 manner and soak in a 25 per cent, solution of glycerin. Use 

 a glycerin-solution in the bottom of the tube. 



Hay-infusion. — 10 g. of chopped hay are macerated with 

 1000 c.c. of water in the water-bath for 3 hours. Filter and 

 sterilize in autoclav for 10 minutes at 120°. 



Wine-must. — ^Wine-must is diluted with four times its 

 weight of water. Dissolve 0.5 per cent, ammonium tartrate, 

 macerate in the water-bath for i hour, filter, and sterilize in 

 Arnold for 3 consecutive days. 



Winogradsky's solution (for nitric organisms). — 



Dissolve in water 1000 c.c. 



Potassium phosphate i • 00 g- 



Magnesium sulphate o. 50 g. 



Calcium chlorid o . 01 g. 



Sodium chlorid 2 . 00 g. 



Distribute 20 c.c. in flasks, and add to each flask a small 

 amount of magnesium carbonate. Sterilize in Arnold for 3 

 consecutive days; then add to each flask 2 c.c. of a sterile 

 2 per cent, solution of ammonium sulphate. 



