APPENDIX II 

 STAINING SOLUTIONS 

 Delafield's hematoxylin — 



Hematoxylin crystals 4 g. 



Alcohol 25 CO. 



Ammonia alum S° g- 



Water 400 c.c. 



Glycerin 100 c.c. 



Methyl alcohol 100 c.c. 



A tttm-hematoxylin — 



1. Hematoxylin 2 g. 



Absolute alcohol 100 c.c. 



2. Ammonia alum :s g. 



Water 100 c.c. 



Mix I and 2 and add — 



Glycerin 850 c.c. 



Glacial acetic acid 100 c.c. 



Allow to stand for one month before using. 

 Bismarck hrovm — 



Bismarck brown o • 5 g- 



Water 100 c.c. 



Sajranin — 



Safranin o ■ S g- 



Water 100 c.c. 



Carbolic thionin-blue (NicoUd) — 



Thionin-blue i g. 



Carbolic acid 2.sg. 



Water 100 c.c. 



Alum carmin — 



Alum 2 . 5 g. 



Carmin 1 . o g. 



Water loo c.c. 



132 



