APPENDIX III 



FROST'S CULTURE CHART (MODIFIED)' 



Group 



Name of organism. . i 



Source, habitat, etc 



References . 



Morphological Characters: 



< 



■2 S 



1^ 



Sketches 



Form: 



a. Broth 



b. Agar 



c. Gelatin 



d. Other Media 



Size 



Cell groupings and arrangements in 



growths 



Staining powers 



a. Anihn-gentian-violet 



b. LoeflBer's methylene-blue 



c. Gram's stain 



d. Special stains 



Motility 



a. Character of movement 



b. Flagella stain 



Spores 



Special characters, such as 



deposits, vacuoles 



pleomorphic and involution forms, 

 capsules, etc 



Physiological Characters 



1. Relation to temperature 



2. Relation to free oxygen 



3. Relation to other agents, such as dessica- 



tion, light, disinfectants, etc 



■ This represents a model only. Open spaces and lines should be extended for 

 practical use to allow sufficient room for descriptions. 



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