FROST'S CULTURE CHART 



135 



4. Pigment production 



5. Growth in carbohydrate media: 



a. Stab or shake culture 



b. Fermentation-tube : 1. Growth in bulb. ... 2. In closed arm .... 



c. Percentage gas produced in: Dextrose Lactose . . .Saccharose 



After 24 hours. 



After 48 hours. 



Gas formula 



d. 



H 

 CO2 



Reaction in bulb 2. Closed arm 



Acid or alkali production, in litmus milk 



Reduction of nitrates; to nitrites to ammonia 



Indol production; 24 hours 48 hours 4 days 



fecal odor; 24 hours 48 hours 4 days 



Enzjon production ; coagulative proteolytic diastatic . 



Characteristic odor 



Pathogenesis 



