CONTENTS 



XI 



APPENDIX A. STERILIZATION 



Sterilization with Chemicals . 



Sterilization with Heat 



Hot-air sterilization 



Steam sterilization at 100° C 



Steam sterilization under pressure ... 



Partial sterilization — Pasteurization ... 



Sterilization by Filtration 



Sterilization by Light . . 



The Technique of Sterilization for Special Purposes 



Glassware, pipettes, and instruments 



Liquid media and other solutions , 



Gelatin and agar media 



Sugar-containing media . . 



Solid vegetables ... 



Milk .... 



Blood serum . . . 



Soil ... 



Seeds . ... . . . 



Growing higher plants under sterile conditions 



115 

 117 

 117 

 118 

 119 

 120 

 121 

 124 

 125 

 125 

 125 

 126 

 127 

 127 

 127 

 123 

 128 

 131 

 1.34 



APPENDIX B. Handling Stock Cultures . . . 

 APPENDIX C. Making Permanent Preparations 

 APPENDIX D. The Inoculating Chamber 

 APPENDIX E. The Titration of Bouillon . . . 

 APPENDIX F. The Determination of Ammonia . 

 APPENDIX G. The Determination of Nitrate 

 APPENDIX H. The Determination of Nitrite 



138 

 139 

 141 

 142 

 143 

 146 

 148 



APPENDIX I. The Determination of Nitrites BY Trommsdorf's 



Method . . . ... 150 



APPENDIX J. Determination op Total Nitrogen .... 151 

 APPENDIX K. The Determination of Reducing Sugars . . 154 



