CONTENTS vii 



49. Prepaeation of Ashby's Solution 28 



50. Preparation op Nitrogen-Free Media for Culture of 



Symbiotic Nitrogen-Fixing Bacteria . . ... 28 



51. Preparation of Winogradsky and Omelianski's Solution 



for Cultivating Nitrite-Forming Organisms ... 29 



52. Preparation op Winogradsky and Omelianski's Solution 



for Cultivating Nitrate-Forming Organisms ... 29 



53. Preparation op N^geli's Solution . . 29 



54. Preparation of Czapek's Nutrient Solution for the Cul- 



tivation op Fungi . . ... .30 



55. Preparation op Sulphindigotate Bouillon fob Detecting 



Anaerobic Organisms ... . . . . . 30 



56. Methods op Cultivating Anaerobic Bacteria . 30 



57. Preparation op Sterile Water Blanks . . . .32 



SECTION VII. FUNDAMENTAL METHODS USED IN THE 

 STAINING AND EXAMINATION OF BACTERIA 



58. Preparation of Simple Stains . . . 33 



59. General Method of Making Stained Preparations . 34 



60. Preparation of Contrast Stains . 35 



61. Special Stains .... . . . . ... 36 



62. Differential Staining . . . . .37 



SECTION VIII. FUNDAMENTAL METHODS OF ISOLATION 



63. Isolation op a Pure Culture 39 



SECTION IX. OUTLINE FOR THE ROUTINE CULTIVATION 

 OF BACTERIA 



64. Preliminary Examination . . . . .... 41 



65. Study of Bacterial Characters . 41 



SECTION X. BACTERIA OF THE AIR 



66. Relation op Bacteria to Atmospheric Dust 48 



67. Determination of the Number op Bacteria in Air . 48 



68. Study of Micrococcus candicans 50 



69. Study of Sarcina lutea . ... 50 



70. Stddy op Bacillus fluorescens LiQUEFACiErrs 50 



71. Study of Bacillus prodigiosus 50 



72. Study of Bacillus subtilis (Hay Bacillus) 50 



