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A MANUAL OF BACTERIOLOGY 



flask 2 from flowing over into flask 1 while sterilization is going 

 on. Wrap flask 1 with paper and place the entire apparatus, includ- 

 ing cylinders for seed, in the autoclave and sterilize for two hours 



Fig. 45. Apparatus for growing seedlings under sterile conditions 

 (After Sohulow) 



A, glass cylinder; B, glass tube 1 cm. in diameter; C, glass rods; D, wire net; 1, 



arrangement at start ; 2, arrangement after three to four days ; 3, arrangement 



after six to eight days 



at 2 atmospheres steam pressure. After the vessel is cool, seal all 

 stoppers with a wax prepared by melting together equal weights of 

 beeswax and rosin. 



The apparatus is now ready for planting. In order to do this 

 under conditions as free as possible from all infection, carry the pots 

 to inoculation room or to some place where the air is quiet and free 



