APPENDIX A 137 



from dust. Remove the cotton plug from the top of the inner glass 

 tube (Fig. 46, -B) and, using sterilized forceps, transfer the seeds 

 germinated under sterile conditions to the apparatus, allowing them 

 to drop down the inner tube on to the wire netting (Fig. 45, D). 



When the young shoot appears after six to eight days, the inner 

 tube B should be slightly raised and the rods C, C used to push the 

 lower mass of cotton gently but firmly around the base of the shoot, 

 as shown in Fig. 45, 2. Finally the tube B, rods C, C, and the 

 upper mass of cotton are removed, leaving the seedling as shown 

 in Fig. 45, 3. 



