166 A MANUAL OF BACTERIOLOGY 



Limits of size Size of majority 



r Orientation (grouping) 



Agar hanging block i Chains (number of elements) 



[ Orientation of chains, parallel, irregular. 

 Location of endospores, cerdral, polar 



3. Endospores 



Form, round, elliptical, elongated. 



Limits of size Size of majority 



Wall, thick, thin. 



Sporangium wall, adherent, not adherent. 



Germination, equatorial, oblique, polar, bipolar, by stretching. 



4. Flagella.No Atta.chjiient,polar,bipolar,peritrichiate. Howstained 



6. Capsules, present on 



6. Zoogloea, Pseudozoogloea. 



7. Involution fonns, on In days at °C. 



8. Staining reactions 



1:10 watery fuchsin, gentian violet, carbol-fuchsin, Loffler's alkaline 



methylene blue. 

 Special stains 



Gram Glycogen 



Fat Acid-fast 



Neisser 



II. Cultural Features (2) 



1. Agar stroke 



Growth, invisible, scanty, moderate, abundant. 



Form of growth,^K/on?i, echinulate, beaded, spreading, plumose, arborescent, 



rhizoid. 

 Elevation of growth, flat, effuse, raised, convex. 

 Luster, glistening, dull, cretaceous. 

 Topography, smooth, contoured, rugose, vemicose. 

 Optical characters, opaque, translucent, opalescent, iridescent. 



Chromogenesis (7), 



Odor, al>sent, decided, resembling 



Consistency, slimy, butyrous, viscid, membranous, coriaceous, brittle. 

 Medium, grayed, browned, reddened, blued, greened, 



2. Potato 



Growth, scanty, moderate, abundant, transient, persistent. 



Form of growth, filiform, echinulate, beaded, spreading, plumx)se, arborescent, 



rhizoid. 

 Elevation of growth, ^ai, effuse, raised, convex. 

 Luster, glistening, dull, cretaceous. 

 Topography, smooth, contoured, rugose, verrucose. 

 Chromogenesis (7), Pigment in water, insoluble, soluble ; other 



solvents, 



Odor, absent, decided, resembling 



Consistency, slimy, butyrous, viscid, membranous, coriaceous, brittle. 

 Medium, grayed, browned, reddened, blued, greened. 



