542 MR. J. B. LAWES, DR. GILBERT, AND DR. PUGH ON 
APPENDIX. 
Received subsequently to the reading of the paper. 
A.—Preparation of solutions for manuring the Plants, dates of application, 
and quantities applied. 
Sulphate-of-Ammonia solution.—Ordinary ammonia-water was distilled from a flask, 
the vapour condensed in a receiver containing pure distilled water, and the strength of 
the solution determined by the volumetric method, by means of dilute sulphuric acid of 
known strength, the preparation-of which is described further on, at p. 545. A given 
volume of the ammoniacal liquid thus prepared was neutralized by pure dilute sulphuric 
acid, of which the quantity added was determined by measurement, and the strength of 
the solution calculated accordingly. It was intended that each cubic centimetre should 
supply about one-tenth of a milligramme of combined nitrogen. The exact strength of 
the sulphate-of-ammonia solutions used in the course of the experiments was as under :— 
TABLE I. 
When used. 
Volume of the pipette 
measure employed. 
Combined nitrogen in a 
pipette measure of the 
solution. 
septems*, gramme. 
In the experiments of 1857 . cmineaiota 111°2 0:00578 
In the experiments of 1858, to “August 10 inclusive ......... 1000 0-004 
In the experiments of 1858, after August 10. 100°0 0:00359 
Tables II. and III. show the dates of the application of the above solutions to the 
different plants, and the amounts of nitrogen so supplied. 
TaBLe IJ.—Showing the supply of combined Nitrogen, as Sulphate-of-Ammonia solution, 
to plants grown in 1857. 
Nitrogen supplied. 
Dates. Wheat, in i i i 
in pre- | Wheat, in pre- | Barley, in pre- | Barley, in pre- 
pared soil. pared pumice. pared soil. pared pumice. 
gramme. gramme. amme. amme. 
June 10...... 00578 00578 00578 “00378 
July 4.0... 00578 *00578 00578 00578 
July 11.0... 00578 00578 00578 *00578 
duly 22 scswes 00578 00578 00578 00578 
July 29...... 00578 00578 00578 
Total ...... 02890 *02890 02890 02312 
* A septem measure is that of 7 grains (=y,55 of a pound avoirdupois) of water; that is, rather less 
than half a cubic centimetre, which is equal to 1543235 grains (or 1 gramme) of ‘cities 
