THE SOURCES OF THE NITROGEN OF VEGETATION, ETC. 573° 
May 22.—A pipette-ful of the sulphuric acid solution added. 
May 29.—One plant dead ; another sickly ; the third has given off shoots 3 to 4 inches 
high, which appear healthy. A pipette-ful of the phosphate-solution added. 
June 7.—A pipette-ful of the sulphate-of-ammonia solution (=0-004 gramme N.) 
added; also a pipette-ful of the phosphate-solution. 
June 19.—Two plants dead; the third has two green shoots, 3 and 5 inches high, 
but delicate. \ A 
June 26.—The two shoots growing languidly ; seven leaves each; also some leaflets. 
July 3.—A pipette-ful of the phosphate-solution added. 
July 14.—Main shoot 12 to 13 inches high ; four lower leaves dead, four upper green ; 
two shoots at the base dead, and one green and thriving. 
July 29.—The two dead plants entirely prostrate, and near one a pale-green moss 
formed on the surface of the soil. The third plant has three dead branches, and one 
about 6 inches high with green leaves at the top. 
August 17.—All the plants dead. 
August 24.—Plants taken up, and submitted to analysis, although the growth so unsa- 
tisfactory. Of course, of themselves, the results can have little weight in reference to 
the question at issue. 
No. 14.—Clover (1858) ; 226 seeds; prepared soil; with nitrogenous manure. 
Two pots of Clover had been sown at the same time as the other plants, one to be 
without, and the other with nitrogenous manure. They came up well, but very soon 
died; and on June 6 two more pots were sown in the same manner as before, excepting 
that the ash was neutralized with sulphuric acid, and re-ignited before being mixed with 
the soil; both also had a pipette-ful of the phosphate-solution, and the one that was to 
have nitrogenous manure a pipette-ful of the ammonia-solution, at the time of sowing 
the seed. The one sown without the ammonia-solution failed so soon that the experi- 
ment was abandoned early; the other (with the ‘ammonia) forms the subject of the 
following record. ‘ 
June 6.—Seeds set as above, in soil with neutralized ash, and both phosphate and 
sulphate-of-ammonia solution added*. 
June 19.—A good deal up; actively and healthily growing. 
June 21.—Second pipette-ful of the sulphate-of-ammonia solution added *. 
June.26.—Third pipette-ful of the sulphate-of-ammonia solution added. Plants quite 
green and healthy. 
-. July 3.—Fourth pipette-ful of the sulphate-of-ammonia solution added; also a pipette- 
ful of the phosphate-solution. 
July 12.—Fifth pipette-ful of the sulphate-of-ammonia solution added. 
July 14.—Sixth pipette-ful of the sulphate-of-ammonia solution added. Surface of 
* It is not quite certain that the ammonia-solution was added at this date; and in the’ Table of the 
results (XIV. p. 531) it is assumed that it was not. 
