28 BACTERIOLOGICAL AND ENZYME CHEMISTRY 



small quantities of material from one medium to another, 

 that is for purposes of inoculation, short lengths of platinum 

 wire mounted in glass rods as in Kg. 6 a are used; for 

 small quantities of liquid a wire with a small loop at the end 

 is employed. With a little care loops can be made which will 

 take up almost exactly a milligram, that is 0-001 c.c. of hquid. 



Fio. 6. — (a) Method of Inoculating the Cultueb Medium; 

 (6) FrsiNQ. 



For transferring colonies of bacteria a small hook is made 

 at the end of the wire. To inoculate a test tube of gelatine 

 the cotton-wool plug is first sterilised by singeing in the 

 Bunsen flame, is removed by a pair of forceps similarly steri- 

 lised, and held between the first and second fingers of the 

 left hand, while the test tube is held between the first finger 

 and thumb (Fig. 6 a). The platinum wire, after having 



