TUBERCULOSIS 83 



1. Zii'ld-Xcdscn method. One grm. of fuchsin is dis- 

 solved in 10 c.e. alcohol and 100 c.e. 5 per cent, plienol 

 water and the solution is filtered. By means of a 

 pipette, a liberal quantity of this staining fluid is 

 dropped on the cover glass which is held by pincers 

 above a flame a couple of minutes until the fluid 

 1 toils briskly, but witlioiit drying. Then the cover glass 

 is rinsed with water and treated for a few seconds 

 with '17} per cent, sulphuric acid and again rinsed with 

 water. If the red color is still distinct, the treatment 

 with acid is I'epeaced and the cover glass is rinsed 

 carefully w^ith a little alcohol. Finally, a few drops of 

 watery solution of methylene blue aic dropped upon it, 

 after whicli it is again rinsed witli water an<l mounted. 



1^. Kitt's method. To 100 c.e. saturated auilin water 

 are added 1 c.e. of a 1 per cent, sodium hydrate solution 

 and 4 to 5 grm. of fuchsin. < )i', the above mentioned 

 phenol-fuchsin solution may be used. The co\'er glass is 

 treated as described above, then rinsed and dijipcd for 

 one-half minute in a fluid consisting of .lO c.e. alcohol, i^H 

 c.e. water and 20 c.e. niti'ic acid, to which has been 

 added as much methylene blue as the fluid will dissolve. 

 Finally, the slide is rinsed with water. 



?<. Cziipleirski's metliad. The staining is done by 

 heating with phenol-fuchsin solution as given above. 

 Allow the staining fluid to run off and, without rinsing, 

 dip the cover glass six to ten times into a concentrated 

 alcoholic solution of fluorescein (1 grm. in 100 c.e. alco- 

 hol). The double staining comes from repeatedly 

 dii^jiing the cover slips in concentrated alcoholic solution 

 of methylene blue (5 to 100) ; then they are rinsed in 

 water. By this method, a decoloration of tubercle bacilli 

 is avoided with certainty. 



In all cases, the preparation is mounted and ex- 



