ELEMENTARY BACTERIOLOGY LABORATORY EXERCISES 5 
SECTION 1: MORPHOLOGY OF MICROORGANISMS 
Exercise 1 
THE MICROSCOPE 
Make a detailed study of the microscope and note carefully its important 
features and its operation, as demonstrated and explained. Also study the book- 
let on “The Microscope”, published by the Spencer Lens Company. For a more 
comprehensive study of the microscope and its uses see “The Microscope”, by 
Gage. 
EXERCISE 2 
MICROSCOPIC EXAMINATION OF INANIMATE OBJECTS 
Make microscopic studies and drawings of the following materials: 
(1) Particles of fine sand 
(2) Crystals of sodium chloride 
(3) Cotton fibers 
(4) Wool 
(5) Fat globules of milk 
Examine the sand particles and salt crystals in the dry state, using the low 
magnification (16 m.m. objective) of the microscope. After observing the salt. 
crystals using the low power, examine them under the 4 m.m. objective. 
Using wet preparations, examine the cotton and wool under the 16 m.m. 
and 4 m.m. objectives. 
Dilute some whole milk with water, using about one part of milk to five 
parts of water. Examine this liquid under the 16 m.m. and 4 m.m. objectives. 
Also observe the Brownian movement of the globules. 
EXERCISE 3 
EXAMINATION OF STAINED BACTERIA 
Study and make drawings of prepared slides showing different morpho-, 
logical types of bacteria. Examine under the oil immersion objective. Stained 
slides of the following organisms are furnished: 
(1) Staphylococcus aureus (5) Bacillus anthracis 
(2) Streptococcus pyogenes (6) Vibrio cholerae 
(3) Sarcina lutea - (7) Spirillum rubrum 
(4) Bacterium typhosum (8) Actinomyces bovis 
