18 ELEMENTARY BACTERIOLOGY LABORATORY EXERCISES 
EXERCISE 29 
INTERMITTENT STERILIZATION 
Sterilize some tubes of broth in flowing steam (100° C.) by the intermittent 
or discontinuous method. Heat for 30 minutes on each of three successive days, 
allowing them to remain at laboratory temperature between heatings. For a 
more complete description of this process, and the principles upon which it is 
based, see “Bacteriology” by Buchanan, pages 105 and 106. 
