62 



Liver : On section a glazed reddish tint, hard, gall-bladder normal, 

 contents yellowish green in colour. 



Spleen : Slightly enlarged ; pulpae soft. 



Stomachs : Abomasum — Mucous membrane patchy, hyperaemia. 

 Rumen — Full of pulpy ingesta, mucosa normal. 

 Reticulum — Contents pulpy, ingesta, mucosa normal. 



Small intestines : Jejunum ; mm. hyperaemia in streaks. 



Ileum ; mm. hyperaemia shght, in streaks. 



Large intestines : Caecum normal. 



Colon ; mm. hyperaemia. 



Kidneys : Eight, normal. 



Left — A whitish coloured body, size of a pea, resembling an 

 infarct. 



Bladder : Normal, contents normal. 



Microscopical Examination of Blood Smears. 



When the blood of an animal sick with East Coast fever is micro- 

 scopically examined after colouration by the method of Giemsa, or other 

 Romanowsky modifications, parasites will be found to make their appear- 

 ance in the globules a few days after a rise of temperature occurs ; it will 

 be observed that the parasites assume various shapes, and that a visible 

 portion of each (nucleus) takes the chromatin stain (red), the remainder 

 (protoplasm) taking the basic stain. The shapes observed will be as 

 follows, viz. : — 



Bacillary ring, ovoid (there appears to be a transitional form between 

 the bacillary and ovoid forms). The average measurement of a number 

 of the hacillary forms was found to be 1 " 5fi in length. They present the 

 form of a baton with the chromatin concentrated at one extremity. In 

 some cases the chromatin occupies about one-half of the total length of 

 the baton. The cytoplasm may be of the same thickness throughout 

 and straight — clove forms, or it may taper to a point at the extremity 

 opposite the karyosome and be curved — comma form. The ring forms 

 average about 1^ in diameter ; they have a clear central portion 

 (vacuole), and the disposition of the chromatin varies. The ovoid 

 forms have hkewise a central clear portion, their length being 1'5/i 

 and their thickness 0" 75,14. 



I have noted that the preparation of the smear during life exercises 

 an influence on the size of the piroplasm, viz., when the blood corpuscles 

 are much contracted the piroplasms are also found reduced in size. The 

 cause of this phenomenon is in all probability the increase of osmotic 

 pressure in the periglobular hqrdd due to a certain evaporation which 

 takes place in making the smear. 



