70 



(5) Number of marks oE all 10 tubes togetlier=!S (total of liaemo- 

 h'sis degrees). 



(a) and {h) multiplied = o x8=40. 



This figure divided by tlie number of horses ^Yhose blood was 

 used (in this example 10) gives the degree of haemolysis 



for 10 horses =^ = 4. 

 Again divided by the number of horses whose blood was 

 used, gives the average degree of haemolysis for 1 



horse = ^^=0"4:. 

 0-4 is the haemolytic index for the serum in question. In 



this way it was possible to compare the results of different 



haemolysis tests. 

 I may mention that for the purpose of drawing conclusions, 



<")nly those tests in which the blood of at least 10 different 



horses were tested with one serum, have been utilised. 



II.— ISOLYSINES IN EQUINES. 

 A. — Hyperimmuxisation by SuBCFTANEors Injection of the Virus. 

 {a.) Hypej'iniinnnisatioii n-ith rirulcnt dpfihrinated blood. 



1. 11 horses hyperinnnunised with viralent defibi'inated liorse 



blood. 



2. o horses ., ,, mule blood. 



3. 3 mules .. .. horse blood. 



4. 4 mules .. .. mule blood. 



5. 1 donkey ,. „ horse blood. 



(h) Hypei^immiDiisatiou with rirulent seru)n. 



1. 3 mules hyi^erimmuuised with virulent horse serum. 



2. 4 mules „ „ mule seiaim. 



3. -1 donkeys ,, ,, horse serum. 



4. 3 donkeys ,, „ mule serum. 



(r) Hyperi)}iinu)usatio)i ivitJi virulent hotiillon. 

 (From peritoneal cavity.) 



1. 2 mules hyperimmunised with virulent horse bouillon. 



2. 4 mules „ ,, mule bouillon. 



B. — HYPERIMMFNISATION by iNTRAJUGULiR INFUSION OF THE ViRUS. 



id) Hyperimmnnisatioa ivitli not defihrinated virulent blood by 

 i)nuie<Jiate traiisfiisioii into the jugular vein. 



1. Horses infused with virulent horse blood. 



2. Mules ., „ mule blood. 



It must he kept in mind that the immune serum derived from tlie 

 hyi^erimmanised animals was never injected alone, but as the experi- 

 ments were in connection with horse-sickness, and not haemolysis, a 

 previous .simultaneous or subsequent injection of virus took place, 



