90 



OONCLUSIOK. 



One mule treated with peritoneal exudate of horses and another 

 injected with peritoneal exudate of mules showed haemoly- 

 sines in their serum, thus ij roving that it is possible 

 to produce isolysines hy subcutaneous injection of peritoneal 

 licxuid. 



Summary of Results from Subcutaneous Injection of Blood, 

 Serum, and Peritoneal Exudate of Horses and Mules into 



Equines. 



The above experiments demon sti'ated that in every instance isoly- 

 sines for horses and mules arose. 



(Ij In horses after injections of horse and mule blood- 



(2) In mules after injections of horse and mule blood and serum 



(except 368 for mules). 



(3) In donkeys after injections of horse blood and serum and 



mule serum. 



It was also possible to obtain isolysines for horse and mule blood in 

 mules by subcutaneous injections of horse and mule peritoneal exudate. 



It stands to I'eason that horses would also produce isolysines for 

 horses and mules after injections of horse and mule serum and probably 

 of peritoneal exudate as well. 



These experiments give no indication how soon the isolysines arise, 

 but they allow of a conclusion regarding the length of time a serum 

 preserves its dissolving properties. Generally they were present up to 

 six months after the last hyperimmunisation. In three instances 

 (horses 611, 612, and 627) the serum was no longer haemolytic after two 

 and two and a half months respective!}^, but in two cases (horses 264 and 

 301) it dissolved mule blood ten months, and in one case even fifteen and 

 a half months after the last injection (mule 199). 



In the majority of cases after the injection of mules with horse 

 blood, the isolytic serum was more haemolytic for horse blood. 



In the majority of cases after the injection of mules with horse 

 serum the isolj^tic serum was more haemolytic for horse bloo^. 



In 50 per cent, of cases after the injection of mules with mule serum 

 the isolytic serum was more haemolytic for horse blood. 



After the injection of a donkey with horse blood the isolytic serum 

 was more haemolj^ic for horse blood. 



In the majority of cases after the injection of horses with horse 

 blood the isolytic serum was more haemolytic for mule blood. 



In all cases after the injection of horses with mule blood the isolytic 

 serum was more haemolvtic for mule blood. 



