Quantitative Bacteriological Examination. 35 



EFFECT OF SIZE OF VESSEL 'UPON THE MtJLTIPLICA- 



TION OF WATER BACTERIA DURING STORAGE. 



(WHIPPLE, 1901.) 



' Average of five plates. 



Wolffliugel and Riedel (Wolffliiigel and Riedel, 1886) 

 noted the dependence of this multiphcation on the air- 

 supply, vessels closed with rubber stoppers showing 

 lower numbers than those plugged with cotton. Simi- 

 larly, Whipple found that the multiplication of bacteria 

 was much greater when bottles were only half full than 

 when they were filled completely; and also, as shown 

 in the above table that the size of the bottle markedly 

 influenced the growth. 



An important series of investigations by Kohn (190^) 

 suggests that this phenomenon of multiplication during 

 storage may be due in part to the solution of certain 

 constituents of glass which favor bacterial life, since the 



