ASSIMILATION OF NITROGEN 69 



ing to the equation: CaC2 + 3N = CaCNz + C. The German commercial 

 name of this product in the raw state is "Kalkstickstoff"' and it is used as a 

 nitrogen fertilizer. 



What has been attained by man only after much travail is commonly ac- 

 complished by plants, however, for we now know a number of plants that can 

 assimilate atmospheric nitrogen. 



§5. Fixation of Atmospheric Nitrogen by the Leguminosse.' — All legumes are 

 able to develop normally, producing a rich harvest with a high nitrogen content, 

 without the addition of any nitrogenous compounds to the soil, as the exact 

 studies of Lawes and Gilbert' have shown. If we cultivate some sort of grain 

 or legume for many years in succession on the same field without applying fer- 

 tilizer, the nitrogen content of the crop finally reaches a certain minimum, beyond 

 which it does not alter. Addition of mineral fertilizers without nitrogen is al- 

 most without effect upon the yield of grain, the nitrogen content remaining al- 

 most the same as before. This is entirely different in the case of the legumes; 

 the same mineral fertilizer without nitrogen produces a marked increase in the 

 nitrogen content of this crop. 



Two series of experiments by P. Wagner^ are illustrated in Figs. 44 and 45, 

 one with peas and the other with oats, the experimental conditions being the 

 same in both cases. The containers marked O contained no fertilizer at all, 

 those marked KP contained potassium and phosphoric acid (PO4), and those 

 marked KPN contained potassium, phosphoric acid and nitrogen as nitrate. 

 Comparison of these figures reveals a distinct difierence between the legumes and 

 the grains in their relation to fertilizers. The growth of oat plants is seen to 

 be very slight in the unfertilized culture, and the addition of potassium and 

 phosphoric acid produces no improvement; while the addition of these together 

 with potassium nitrate produces excellent growth (Fig. 44). The behavior of the 

 pea plants is entirely different. These do not need nitrate fertilizer, addition 

 of potassium and phosphoric acid being sufficient to produce normal growth. 

 In this case the total need of nitrogen is supplied from the air (Fig. 45). 



The results of Lawes and Gilbert and those of Wagner thus seem to dis- 

 agree with the conclusions reached by Boussingault (see page 60). This is 

 explained by the fact that Boussingault used sterilized soils, whereas the other 



1 Frank, A., Die Nutzbarmachung des freien Stickstoffs der Luft fur Landwirtschaft und Industrie. 

 Zeitsch. angew. Chem. i6: 536-539. 1903. Gerlach, M., Die Nutzbarmachung des atmospB&rischen 

 Stickstoffes. lUustr. landw. Zeitg. 1904. Nos. s and 7.* Review by Vogel in; Centralbl. Bakt. //, 12 : 

 495-497. 1904. [See also review in; Exp. sta. rec. IS: 25. 1903-04.] 



2 Wagner, P., Ergebnisse von Dtinungungsversuchen in Lichtdruckbildern mit erlauterndem Vortrage 

 fiber die rationelle Diingung der land wirtschaf tlichen Kulturpflanzen. 2te Aufl. Darmstadt, 1 891. 



' Lawes, J. B., and Gilbert, J. H., The sources of the nitrogen of our leguminous crops. 

 Jour. Roy. Agric. Soc. England ///, 2: 657-702. London, 1891. Idem, The Rothamsted 

 memoirs on agricultural chemistry and physiology. 7 v. London, 1886-1899. Idem, same 

 title. 3 V.London, 1 890-1 893. Hall, A. D., The book of the Rothamsted experiments. 294 p. 

 New York, 1905. For a brief discussion of this whole matter see: Russell, E. J., Soil condi- 

 tions and plant growth. London, 1915. Page lo et seq., also page 80 et seq. Russell's ex- 

 cellent bibliography includes references to a number of the' papers of Lawes and Gilbert. 

 These papers have all been collected and published in the Rothamsted Memoirs, and 

 Lawes and Gilbert's results are summarized by Hall. — Ed. 



