40 PROTOPLASM 



After the last measurement on 29 th May, at 10 A.M. (see 

 Table II.), the preparations were thoroughly washed out with 

 semi-dilute glycerine, and then left to stand in this fluid. 

 After about an hour the drops had already become much 

 smaller. Since the 'drops e, and / flowed together after a 

 short time, this preparation was not further taken into 

 account. The decrease of volume was further maintained, 

 so that the diameters of the three drops of preparation I., 

 which had again become completely round, were, at 9.30 

 A.M. on the 30 th, about as follows : — 



III— a =0-899 

 & = 0-.572 

 c = 0-668 



The glycerine was then washed out with water ; the increase 

 in size of the drops was quite visible in an hour's time, and 

 their diameters on the following day, 1st June, at 11.30 A.M., 

 had attained the following dimensions : — 



IV.— a =1-747 (m) 

 &= 1-285 \m) 

 c= 1-567 (m) 



As results from a comparison with Table II., the dimen- 

 sions of all three drops are now somewhat larger than at 

 first, which no doubt depends on the fact that originally 

 they were not formed in pure water, but in a weak KgCOg 

 solution, which, like glycerine, naturally exerts an osmotic 

 effect. 



Finally, on the 1st June, the water was once again 

 replaced by half-diluted glycerine. The drops now adhered 

 somewhat strongly to the glass, so that with the contraction 

 that took place they became more or less sausage shaped. 

 Their diminution in size was again speedily very striking, 

 and by the following day, 2nd June, at 10.15 A.M., they 

 shrank to the following dimensions : — 



v.— ft =0-937 (m) [ + 0-038' 

 6 = 0-609 (m) [ + 0-037" 

 c= 0-673 (m) r + 0-005 



