FLAGELLATA AND RADIOLARIA 91 



other respects to the protoplasm, is more finely meshed and 

 coloured a stronger blue, and also -that numerous intensely 

 red granules are lodged in the nodal points. These granules, 

 which correspond to the chromatin granules observed by me 

 (1890) in numerous animal and vegetable nuclei by means 

 of the same reaction, are much smaller than those above 

 described in the protoplasm, and stain much more strongly. 



Flagellata 



Only a few have been occasionally studied by me. In 

 living Chilomonas paramcecium (Plate V. Fig. 3, hinder end) 

 I could observe with perfect distinctness the radially 

 striated alveolar layer under the rather thick and dark 

 pellicle (p), as well as the reticular structure of the adjacent 

 internal protoplasm. Preparations which were made with 

 osmic acid or iodine -alcohol, and stained in Delafi eld's 

 haematoxylin, showed the alveolar layer and the reticular 

 structure of the internal protoplasm in just the same 

 manner, and in particular again permitted the determina- 

 tion of the fact that the meshes surrounding the nucleus 

 are directed radially to its surface. The alveolar layer 

 and reticular structure were also observed with just the 

 same degree of perfection in preparations of Gryptomonas. 

 In the same way too, in preparations of Euglence which I 

 studied casually, a relatively thin but distinct alveolar layer, 

 and a reticular endoplasm, frequently filled with larger 

 vacuoles, could be well seen. The results mentioned 

 harmonise well with the structural relations of these and 

 other forms of Flagellata which have been observed recently 

 (1889) and quite independently by Kiinstler. 



As I have already remarked elsewhere (1890), there 

 are to be found in the endoplasm of Flagellata, when they 

 are killed with alcohol or iodine-alcohol and cautiously 

 stained with Delafield's hcematoxylin, larger or smaller 

 quantities of small granules stained an intense red. They 

 have been as yet especially observed in Chilomonas, 

 Gryptomonas, E%gUna, Lepocinclis, and Trachelomonas, and 

 agree closely both in size and colouring with the chromatin 



