15 



This is less refractile in the central part, presents a finely 

 granulose structure, and envelops the central part like an involucre. 



Finally, so far as concerns the relation botvi/een the central part 

 and the peripheral part, I observed that in the fixed and stained 

 preparations the first is almost always separated from the second by 

 means of a colorless halo (PI. XIII, fig. 9, a). 



Staining Methods , In the examination of the body under consideration 

 I find fresh material desirable or fixed material treated with different 

 reagenl^s. As fixatives I have employed absolute alcohol containing 5 per 

 cent of acetic acid, platinum chlorid«'plus acetic acid (Merkel) , satur- 

 ated solution of corrosive sublimate, acetic acid plus osmic acid plus 

 platinum chloride (Hermfinn), Flemming's solution,- Eabl^s solution, Keiser'a 

 solution, chromic acid, alcoholic or aqueous solutions of picric acid, osmic 

 acid, 



imong these I have obtained the best results with the f irjgt four. 



VJith regard to the stains, without enumerating all the reagents which 

 I used, I note here only t^ose which have givuin good results, 



¥/ith Flemming*s triple stain (safranine plus gentian violet plus 

 Orange g.) the nucleoli and the bodies stained red and the chromatin of the 



nucleus violet, . .. 



V\fith the triple stain Ehrlicb-Biondi-Heidenhain, used on material fixed 

 With absolute alcohol plus 5 per cent acetic acid, the nucleus and the body 

 stained intensely red, 



V/ith acid fuchsine, eosine, erythrosine, and aurantia, I obtained a 

 beautiful stain of the body and of the nucleus. V/ith such stains it is^topbe 

 noted that the central part of the body stains more intensely than the 

 peripheral part, 



