- 12 - 

 Similar cellular bodies containing anthocyanine are fo-und in the 

 flowers of Ne-peta pilechoma . 



Weigela rosea Lindl., W. .japonica Thionb, 



With an idea of getting an exact conception of the method by which the 



rbd color forms in the flowers of this species, I have studied many specimens 



and have found that they are not derived from preexisting substances soluble 



in the cell sap but have organized themselves into special spherical bodies 



immersed in the cytoplasm. These, being at first hardly visible, increase in 



size little by little and later, having reached a definite size (PI. XVII, fig, 



■f- 

 5), degenerate and dissolve completely, coloring the cell sap, which was at 



first colorless. 



According to the data obtained from reactions which I got, the pigment 

 of such body belongs to anthocyanine. It is contained with the membranous in- 

 volucre the presence of V7hich may be demonstrated by application of the usual 

 methods described above, and particularly by using oxygenated water, 



Conval lar ia .iaponica Linn. 



In this species the cyanoplasts are found in process of formation in 

 the epidermal and subepidermal cells of the adult fruit and, above all, in 

 those of the ultimate strata of the seed coat in contact with the seed. Here 

 the cyanoplasts in the adult stage are spherical, relatively small, immersed 

 in the cytoplasm, and situated in an indeterminate position (PI. XVIII, f ig.2) , 



They are at first present as very minute spheres azure blue in color 

 (PI. XVIII, fig. 1), or as colorless and refractile bodies which give all the 

 reactions peculiar to tannin. 



Such spheres grow, asstime an inttose coloration, in the end dissolve 

 and color the cell sap, which f^ the first to this time has been peiffectly 

 colorless (Pl.XVIII, fig. 3). 



