- 3 - ' 



IS often ooserved in its interior some gr.^nviles or nvirnerous little spheres due 



to a precipitation. These granules dissolve in water and absorb many aniline 

 dyes. 



With alkaloids (caffeine, chinine, nicotine, morphine, cocaine, atropine, 

 strychnine, and codeine), in 2 per cent solutions, one obtains sometimes a 

 scant precipitation similar to that obtained with the carbonates. 



Methylene blue in aijueous solution is absorbed with avidity and commu- 

 nicates to the body, after a short time, a beautiful color. 



Examining the reactions alluded to, we reach the following conclusions: 

 In Sudan III, Scarlet E, and tincture of Alkanna, we did not get the 

 characteristic color for oleose substances. 



Tha reactions obtained with a solution of iodide of potassium, with 

 Millon's reagent, and with Trommer's reagent are the same as those #iich in 

 the microchemical examination serve to show proteinaceous substances. 



Osmic acid, ferric salts, bichromate of potash, alkaline carbonates, 

 alkaloids, and ro.ethylene blue behave precisely as in the presence of tannin. 

 From this complex of reactions one may conclude that within the body 

 under examination there exist proteinaceous and tannic substances, while oleose 

 substances are lacking. 



Colorization . In some works by Pfeffer (l, 2) a/ rnay be found refer- 

 ences to the absorption of aniline dyes by the living cell. It is there to 

 be noted also that a color substance incapable of osmosis may be contained in 

 the cell sap because of the presence of diverse bodies. From these are noted 

 to date fluoroglucin and tannin. This latter, according to Pfeffer, may be 

 determined by any of all of the absorbable aniline dyes except rosolic acid. 



Having established by means of many reagents the presence of tannin in 

 the body under examination, I believed it worth while to check the observations 

 of Pfeffer, using, in addition to the colors used by him, some others. 

 a/ Reference is made by number to "Literature cited," near end of translation. 



