- 6 - 



In ^vater at ordinary temperatiore , it varaolizes but does not disappear. 



In saline solutions of sodium chloride, potassium sulphate, and am- 

 monium chloride, after 24 hours, it appears as a vacuole bounded by a thick 

 wall. 



This is not soluble in alc&b4l,nor in acetic acid, sulphuric acid, 

 nor dilute hydrochloric acid. 



With Millon's reagent heated ever so little, one obtain^s a more de- 

 cided reaction; in fact, Immersing the tangential sections taken from the 

 ventral or dorsal surfaces of the leaf blades in a drop of the reagent, the 

 body, retaining its original form, atssumec;. -a reddish color which, after slow 

 TAiarming of the preparation, becomes intensely bridk red. 



With especially rich coloring sub stance(quino line, Sudan III, Scarlet 

 E, and sulphate Nilblau) , one obtains no color reaction. One obtains, on 

 the contrary, an important reaction by using 1 per cent osmic acid. Treating 

 sections similar to the precedinp; with this reagent, a '.:reat number of the 

 bodies in question turned entirely black, while some showed a black interior 

 part due to tannic substance and an external peripheral colorless part which 

 is refractile. This, with solutions of potassium iodide, stains brownish 

 yellow, shows all the other special reactions of proteinaceous substance, 

 and is, therefore, to be considered as forming a plasmatic involucre in which 

 tannic substance is included. Such an involucre, in preparations fixed in 

 alcohol and then treated with a mixture of fuchsine and methyl green, appears 

 granulose and distinctly red in color. 



The body under examination, moreover, because of the. tannin which it 

 contains .turns reddish brown* with bichromate of potash in concentrated so- 

 lution, bluish black in iron acetate and iron chloride, bro\'«n in 1 per cent 

 chromic acid, yellow when treated with alkali and with alkaline carbonate. 

 It in fact, absorbs many of the aniline colors without any previous fixation. 



