vi CONTENTS 



CHAPTER V. 



Preparation of Media — Bouillon, Gelatin, Agar-agar, Potato, 

 Blood-serum, Blood-serum from Small Animals, Milk, 

 Litmus-whey Milk, Durham's , Peptone Solution, Lactose 

 Litmus-agar, Loffler's Blood-serum Mixture, the Serum- 

 water Media of Hiss, Guarniari's Gelatin-agar Mixture . . 108 



CHAPTER VI. 



Preparation of the Tubes, Flasks, etc., in which the Media are to 



be Preserved . ... 133 



CHAPTER VII. 



Technique of Isolating Bacteria in Pure Culture by the Plate and 



the Tube Method . . 137 



CHAPTER VIII. 



The Incubating Oven — The Safety Burner Employed in Heating 



the Incubator — Thermo-regulator — Gas-pressure Regulator 145 



CHAPTER IX. 



The Study of Colonies — Their Naked-eye Peculiarities and Their 

 Appearance Under Different Conditions — Differences in the 

 Structure of Colonies from Different Species of Bacteria — ■ 

 Stab-cultures — Slant-cultures 152 



CHAPTER X. 



Methods of Staining — Cover-slip Preparations — Impression 

 Cover-shp Preparations — Solutions Employed — Preparation 

 and Staining of Cover-slips — Staining Solutions — Special 

 Staining Methods . 158 



CHAPTER XI. 



Systematic Study of an Organism — Points to be Considered in 

 Determining the Morphologic and Biologic Characters of a 

 Culture — Methods by which the Various Biologic and Chem- 

 ical Characters of a Culture may be Ascertained — Dark 

 Field Illumination — Facts Necessary to Permit the Identifi- 

 cation of an Organism as a Definite Species 179 



