CONTENTS. 



INTRODUCTION. 



"Omne Vivum ex Vivo"— The Overthrow of the Doctrine of 

 Spontaneous Generation — EarUer Bacteriological Studies — 

 The Birth of Modern Bacteriology . 17 



CHAPTER I. 



Definition of Bacteria — Differences Between Parasites and 

 Saprophytes — Their Place in Nature — Bacterial Enzymes 

 — Products of Bacteria — Nutrition of Bacteria — ^Their Rela- 

 tion to Oxygen — Influence of Temperature Upon Their 

 Growth — Chemotaxis ... . . 31 



CHAPTER II. 



Morphology of Bacteria — ^Chemical Composition of Bacteria — 



Mode of Multiplication — Spore-formation — Motility . . 60 



CHAPTER III. 



Principles of SteriUzation by Heat — Methods Employed — Dis- 

 continued Sterilization — Fractional Sterilization — Apparatus 

 Employed — Sterilization under Pressure — Sterilization by 

 Hot Air — Thermal Death-point of Bacteria — Chemical Dis- 

 infection and Sterilization — Mode of Action of Disinfectants 

 — Practical Disinfection . . 73 



CHAPTER IV. 



Principles Involved in the Methods of Isolation of Bacteria in 

 Pure Culture by the Plate Method of Koch — Materials 

 Employed 101 



(V) 



