PUS FROM ACTINOMYCOSIS. 27 



Pus from Actinomycosis. 



Note the minute greyish yellow granules in the pus. 



5. Place on a slide a drop of the pus, containing 

 one or more granules. Treat it with several drops 

 10% caustic potash solution and press on a cover glass. 



Examine with low and high dry lenses. Radiating 

 masses of the fungus and separate threads can be readily 

 detected. 



6. Prepare cover glass films from the pus, and stain 

 by Weigert's modification of Gram's method. 



(a) Place the films in alcohol 1 to 2 minutes, 

 (fe) Transfer to anilin gentian violet, 10 to 15 

 minutes. 



(o) Rinse in water and then transfer for 2 to 3 

 minutes to Weigert's iodine solution, 



Iodine 1 gramme. 



Potassium iodide ... 2 grammes. 

 Water 100 cc. 



(d) Rinse again in water. Counterstain in eosin 

 for 1 minute. 



(e) Wash in water and remove excess of water with 

 blotting paper. 



(/) Dehydrate and decolorize in a solution of anilin 

 oil 2 parts, xylol 1 part, till violet color has almost 

 disappeared. 



(g) Wash in xylol to remove all anilin oil and then 

 mount in Canada balsam. 



Examine with the low and high dry lenses and with 

 the oil-immersion lens. 



