TUBERCLE BACILLI FROM GUINEA PIG. 37 



The animal is opened in the usual manner, and the 

 enlarged inguinal and iliac glands are removed with a 

 sterile forceps, and broken up in sterile capsules. 



4. From the pulp in the capsules inoculate freely, 

 (a) 6 blood serum tubes; 



(6) 6 glycerine broth tubes; 



(c) 6 glycerine agar tubes. 



After inoculation seal the tubes with ordinary w^x 

 or paraffin, so as to prevent evaporation. 



Incubate at 37°C. and examine at the end of 7, 10, 

 14, and 21 days. 



5. From the culture of Bacillus diphtherise furnished 

 inoculate, 



(a) a blood serum tube; 

 (&) a glycerine agar tube (slope); 

 (c) a broth tube. 



Place in the incubator at 37°C. and examine at end 

 of 18, 24 and 48 hours. 



