48 PRACTICAL BACTERIOLOGY. 



DEMONSTRATION XIX. 



Anaerobic Cultures— Bacillus Tetani— Bacillus CEdematis Maligni — 



Bacillus Capsulatus Aerogenes — Inoculation o£ Animals with 



Bacillus Tetani, Bacillus CEdematis Maligni and 



Bacillus Capsulatus Aerogenes. 



1. Make inoculations in the usual manner of Bacillus 

 tetani, Bacillus oedematis maligni and Bacillus capsu- 

 latus aerogenes on, 



(a) agar tubes (stab) to which .5% formate of soda 

 has been added; 



(6) grape sugar gelatine tubes (stab); 



(c) grape sugar broth tubes. 



Place the broth cultures in Novy's jar, and treat the 

 agar and gelatine cultures by Buchner's method, as de- 

 scribed below. 



Anaerobic Culture Methods. 



Anaerobiosis may be obtained in several ways. One 

 of the most commonly employed methods, particularly 

 when working on a large scale, is to replace the oxygen 

 (air) by hydrogen gas. For culture tubes and plates 

 Novy's jars are very convenient for use with hydrogen. 

 2. The cultures being placed in the jar, hydrogen 

 from a generating apparatus (Kipp's) is allowed to 

 stream into the jar for one hour. The hydrogen is 

 usually purified by passing through a series of wash 

 bottles containing lead nitrate in the first, silver nitrate 

 in second and freshly prepared pyrogallate of potash in 



