16 PRACTICAL BACTERIOLOGY. 



Cultures of Bacillus Anthracis. 

 From the culture tube supplied inoculate, 

 (a) an agar-agar (slope) tube; 

 (6) a potato tube; 



(c) a gelatine (stab) tube; 



(d) a broth tube; 



(e) a broth tube, to which carbolic acid has been 

 added in proportion of 1 to 1000; 



(/) a litmus milk tube. 



Place all except (c) in the incubator at ST^C, and 

 keep (c) at 20°C. 



Examine these growths at the end of 24, 48 and 72 

 hours, noting the characters of growth on the various 

 media. 



