PART I. 

 PRACTICAL BACTERIOLOGY. 



DEMONSTRATION I. 



Inoculation of Culture Media from Pure Cultures of Bacteria. 



Students must remember that they are to work with 

 many of the actual causative factors of disease — the 

 pathogenic bacteria; hence there is a liability to self- 

 infection or infection of the laboratory, unless care is 

 taken to develop a proper technique. The practical 

 work is started with non-pathogenic species, or with 

 species of low pathogenic power, so that such a tech- 

 nique may be acquired without danger. 



The points to be noted by the student are: 



(a) The character of the various culture media. 



(b) The method of holding culture tubes, and re- 

 moving and replacing plugs during inoculation. 



(c) The care, and particularly the sterilization, of 

 the platinum needle or loop, before and after use. 



(d) That minute amounts of the bacterial culture 

 suffice for inoculation purposes. 



(e) The proper labelling and placing in incubators 

 of the inoculated tubes. 



1. Make two agar-agar slope cultures of Bacillus 

 prodigiosus. 



Place one tube in the incubator at 37°C (98.6 F), 

 and the other at 20°0 (69°F). 



