150 MICROBES, FERMENTS, AND MOULDS. 
mixed microbes, presenting no trace of the original 
liquid, and this was done in vessels protected from air- 
germs. These cultures may be carried to the eighth 
generation. 
Asses and horses inoculated with liquid containing 
the microbes produced by this culture have died with 
the lesions characteristic of glanders (glanderous 
tubercles in the spleen, lungs, etc.). Cats and other 
animals which have been inoculated in the same way 
die with glanderous tubercles in the lymphatic glands 
and other organs. 
It follows from these experiments that the microbe 
which causes this disease is always reproduced in the 
different culture liquids with its characteristic form 
and dimensions ; that uni-ungulates can be inoculated 
with it, as well as man and other animals. In fact, this 
microbe is the essential cause of the disease. 
VIII. PeBRINE AND FLACHERIE, DISEASES AFFECTING 
SILKWORMS. 
We have already spoken of muscardine, a silk- 
worm’s disease produced by a microscopic fungus; 
two other diseases are caused by distinct microbes, of 
which we must shortly speak. 
Pebrine—lIn the silkworm nurseries, in which this 
disease prevails, the silkworms which issue from the 
eggs, technically called seed, are slowly and irregularly 
developed, so as to vary greatly im size. Many die 
