LABORATORY RESEARCH, ETC. 265 
The stage of the microscope is maintained at the 
requisite temperature. . 
In order to make cultures in large quantities, other 
kinds of apparatus are in use. The liquid supposed to 
contain microbes is introduced into sterilized nutritive 
liquids by means of a platinum wire, which has been 
heated red hot and then allowed to cool; its end is 
TG: 10 erllzing of tanks, wigaleave tabeas 
dipped into the liquid, and then instantly transferred 
to the culture, while it is exposed to the heat of a 
spirit-lamp. The flask is then sealed with a wad of 
cotton wool. 
The culture liquids employed by Pasteur are the 
extract of beer-yeast, an infusion of hay, boiled and 
neutralized urine, and the broth of various kinds of 
