I08 PROTOZOANS 



Study the nucleus. Position? Shape? Size? Is it in the 

 same relative place in the different specimens ? Drop a small 

 drop of aceto-carmine on the side of the cover glass and 

 watch the effect on the amoeba. Is there more than one 

 nucleus ? 



Find contractile vacuole. It may be known by its pul- 

 sating movement. Position? Shape? Number? Has it 

 any definite contents? What function does the vacuole 

 seem to perform ? Make an enlarged drawing of the amceba 

 naming the parts. 



(3) The Paramecium (slipper animalcule) : Examine 

 as you did the amoeba. How does it compare with the 

 amoeba in size ? In shape ? Does it change its shape during 

 its movement. Find the ciiticle, or cell wall. Note its ex- 

 treme transparency. Is it present over the entire surface? 

 Is it entire or has it openings? 



Find the ectosarc and endosarc and compare with those 

 of the amoeba. Do you find food vacuoles, i. e., particles of 

 food-ingested substances ? 



Do you find contractile vacuoles? How many? Where 

 situated ? Examine several specimens to see if the vacuoles 

 are constant in number and position. Find the nucleus, and, 

 if possible, a micro nucleus beside it. Note size and position. 

 Compare nucleus with that of the amoeba. 



Do you find cilia covering the body? Do they cover the 

 body entire? Why? Examine a single cilinin and draw it. 

 Are the cilia all of the same size and shape? 



The trichocysts are seen directly beneath the cuticle. How 

 are they arranged? What is their shape and structure? 



Do you find the mouth? What is its position? Shape? 

 Is the position constant ? Is the shape of the mouth perma- 



