222 APPENDtX 



Borax Carmine (Grenachcr's). — Solution of powdered 

 carmine and borax in water and alcohol. The beginner 

 should order it in solution, and not dry. It is used for 

 staining, and is perhaps the most serviceable for general 

 use. It may be used cold or at a temperature of 50° C. 



Magenta. — Solution of 34 grm, of roseine in i liter 

 of water, add 5 c.c. absolute alcohol. It is used to stain 

 fresh preparations for immediate and temporary use. 



Oil of Cloves. — A clearing agent. Used to clear a 

 specimen of alcohol before permanent mounting. Should 

 clear the object, but not stay on the slide too long, or it 

 will make the section brittle. 



TuRi'ENTiNE. — May be used as a clearing agent. Also 

 used to remove paraffin from sections before mounting. 



Salt Solution. — Normal solution of 7.5 grms. common 

 salt in liter of water. Used for examining living or freshly 

 killed tissues. 



Glycerine. — Used pure or diluted with water for mount- 

 ing objects for microscopic study. 



Canada Balsam. — The common name for balsam fir 

 of commerce. May be used as bought from druggist or 

 evaporated to dryness, powdered and dissolved in alcohol 

 or turpentine. It is the most commonly used medium for 

 mounting stained sections for microscopic study. 



Paraffin. — Used for imbedding objects for permanent 

 mounting. Paraffin with melting point 56° C. may be used 

 alone or mixed with paraffin the melting point of which is 

 40° C. 



Shell-lac.: — Dry shell-lac dissolved in absolute alcohol, 

 allowed to stand for some time is ready for use. Used to 

 fix the sections to the slide for mounting. 



