30 CANINE DISTEMPER 
tissues, sputum, and pustules, which he cultivated in 
neutral or slightly alkaline broth. Pure cultures were 
made, which, on injection into healthy susceptible dogs, 
set up catarrhal and pustular symptoms accompanied by 
acute pyrexia, and followed sometimes by death of the 
animal. Survivors, however, had acquired an immunity. 
Marcone and Meloni (1888) isolated staphylococci which, 
when cultivated and inoculated into oes: set up pustules 
but no typical distemper. 
Jacquot and Legrain (1890) found in the pustules motile 
micrococci which were from 06 to o'8u* in diameter, 
and which formed diplococci; but inoculations of their 
cultures produced no better results than Marcone did 
with his organism, and brought about no immunity. 
The coccus found by Mathis was thought by Lignieéres, 
Carré, and others to be identical with this one, and it 
was considered to be the direct cause of the pustules. 
Millais (1890) found in lungs, liver, trachea, spleen, and 
nasal mucus a long bacillus which, on inoculation, set 
up typical distemper, and which liquefied gelatin, 
descending as a flaky mass in the almost clear fluid, 
which was covered bya whitish scum. Finally, when the 
whole of the gelatin was liquefied, the flakes gathered at 
the base, and, if stirred, appeared to be of a ropy con- 
sistency. Healso found a micrococcus in great abundance, 
which he thought was the cause of the lung lesions. The 
two combined caused the pneumo-distemper (Veterinary 
Journal,.May, 1890). 
Schantyr (1892) claimed to have found three organisms, 
and divided distemper into three diseases, each produced 
by a distinct organism (see p. 7). 
Jensen (1896) declared that the pneumonia of distemper 
was due toa streptococcus, which he demonstrated. 
Valerio (1896) found in the pustules, spinal medulla, 
* »= microns. 
