PREVENTIVE INOCULATION 81 
‘The committee consider the first experiment an entire 
failure, and the second inconclusive but suggestive. On 
the other hand, they regard the results of the third ex- 
periment as unimpeachable evidence that the vaccination 
failed to confer any immunity against distemper. . . 
The net result of the experiment was that three of the 
four vaccinated pups died of distemper, while only two 
deaths occurred among the four unvaccinated pups.” 
In face of all this adverse evidence, one must reluct- 
antly conclude that the material is valueless as a pre- 
ventive. 
Ligniéres’ Polyvalent Vaccine.—In an article on “ Vac- 
cination against Distemper” printed in the Recueil de 
Medecine Vetérinaire for July, 1903, Professor Ligniéres 
detailed the preparation of his polyvalent vaccine. The 
’ Pasteurella canis is used, and a mixture of its various 
strains is grown on agar-agar, but in order safely to 
diminish their virulence several hundred subcultures 
are carried out. As with the Phisalix preparation, two 
vaccines are employed, the first being subjected to a 
temperature of 42° C. for five days, while the second 
and stronger one is subjected to a like temperature for 
only two days. The dose of either is 1 c.c. No better 
results, however, appear to have been obtained with this 
vaccine than with its predecessor, and it has not yet 
succeeded in establishing itself as a recognised prophy- 
lactic. 
