366 



Hydrophobia, Lyssa, or Rabies 



from the unstructured Negri bodies observed with great frequency 

 in the rabid guinea-pig brain. In a few instances these forms con- 

 tained a blue-staining central ring or point, and closely resembled 

 the structured forms of Negri bodies. The normal guinea-pig brain 

 inoculated with rabid material, street or fixed virus, incubated in the 

 same manner, showed the same structures. The brains of guinea- 

 pigs dying of street virus and rabbits dying of fixed virus, incubated 

 in small fragments, gave no development of Negri bodies in blood 

 plasma, beyond the small structured and unstructured forms, al- 

 though in one preparation the ganglion cells appeared to be living 

 at the end of twenty-one days' incubation. 



Cultivation. — Attempts to cultivate Negri bodies were made by 

 Moon,* but the success of his attempts seemed doubtful. The first 



Fig. 136. — From rabbit " fixed- virus" brain; a, b, c, d,f, and i, tj^pes of Negri 

 bodies seen at deatli of rabbit; c, g, h, and/, apparent multiplication and segmen- 

 tation of the bodies after three days at 24°C. Drawing made from smears 

 stained by Giemsa's method and magnified about 2000 diameters (Williams, in 

 Jour. Am. Med. Assoc). 



claim to successful cultivation of the Negri bodies was made by 

 Noguchi.f The cultivation was done according to his already suc- 

 cessful method for Spirochaeta of various kinds. Large, small and 

 dividing bodies appeared in the culture fluid, after inoculation with a 

 fragment of nervous tissue from various animals with infection fol- 

 lowing inoculation with street virus and "fixed" virus. But Wil- 

 liams! at once pointed out that there is no certainty that the bodies 

 increased in numbers in the cultures, though Noguchi says that they 

 reappear in new cultures "through many generations." Noguchi's 



* "Jour, of Infectious Diseases," igi3, xiii, 213. 



t "Jour, of Experimental Medicine," 1913, xviii, 314. 



j "Jour. Amer. Med. Assoc," 1913, lxi, 1509. 



