Cultivation 



697 



this bacillus upon artificially prepared media, but in 1903 Hansen,* 

 who discovered the organism, declared that no one had yet culti- 

 vated it. 



Bordoni-Uffreduzzif was able to cultivate a bacillus which par- 

 took of the staining peculiarities of the lepra bacillus as it appears 

 in the tissues, but differed in morphology. 



CzaplewskiJ confirmed the work of Bordoni-Uffredozzi, and 



Fig. 283. — Section of one of the nodules from the patient shown in Fig. 285, 

 stained by the Weigert-Gram method to show the lepra bacilli scattered through 

 the tissue and inclosed in the large vacuolated "lepra-cells." Magnified 1000 

 diameters. 



described a bacillus supposed to be the lepra bacillus, which he 

 succeeded in cultivating from the nasal secretions of a leper. 



The bacillus was isolated upon a culture-medium consisting of 

 glycerinized serum without the addition of salt, peptone, or sugar. 

 The mixture was poured into Petri dishes, coagulated by heat, and 

 sterilized by the intermittent method. 



* KoUe and Wassermann's "Handbuch der pathogenen Mikroorganismen," 

 n, p. 184, 1903. 



t "Zeitschrift f. Hygiene," etc., 1884, in. 



t "Centralbl. f. Bakt. und Parasiteuk.," Jan. 31, 1898, vol. xxin, Nos. 3 and 

 4, p. 97- 



