7IO 



Glanders 



Gelatin is not liquefied. The growth upon the surface is grayish 

 white and slimy, never abundant. 



Agar-agar. — Upon agar-agar and glycerin agar-agar the growth 

 occurs as a moist shining viscid layer. 



Blood-serum. — Upon blood-serum the growth is rather character- 

 istic, the colonies along the line of inoculation appearing as cir- 

 cumscribed, clear, transparent drops, which later become confluent 

 and form a transparent layer unaccompanied by liquefaction. 



Potato. — The most characteristic growth is upon potato. It first 

 appears in about forty-eight hours as a transparent, honey-like, 

 yellowish layer, developing only at incubation temperatures, and 

 soon becoming reddish-brown in color. As this brown color of the 

 colony develops, the potato for a considerable distance around it 

 becomes greenish brown. Bacillus pyocyaneus sometimes produces 

 somewhat the same appearance. 



Fig. 287. — Culture of glanders upon cooked potato (Loffler). 



Milk. — In litmus milk the glanders baciUus produces acid. A 

 firm coagulum forms and subsequently separates from the clear 

 reddish whey. 



Metabolic Products. — The organism produces acids and curdling 

 ferments. It forms no indol, no liquefying or proteolytic ferments. 

 There is no exotoxin. All the poisonous substances seem to be 

 endotoxins. 



Mallein.— Babes,* Bonome,t Pearson,! and others have prepared 

 a substance, mallein, from cultures of the glanders bacillus, and have 

 employed it for diagnostic purposes. It seems to be useful in veteri- 



* "Archiv de Med. exp. et d'Anat. patholog.," 1892, No. 4. 



t "Deutsche med. Woch.," 1894, Nos. 36 and 38, pp. 703, 725, and 744. 



I Jour, of Comp. Med. and Vet. Archiv," Phila., 1891, xii, pp 411-415 



